Clone and expression analysis of ThPR1 gene in Tamarix hispida

ZHANG Kaimin,WANG Yucheng, YANG Guiyan, GAO Caiqiu

Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2013, Vol. 37 ›› Issue (02) : 45-49.

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Journal of Nanjing Forestry University (Natural Sciences Edition) ›› 2013, Vol. 37 ›› Issue (02) : 45-49. DOI: 10.3969/j.issn.1000-2006.2013.02.008

Clone and expression analysis of ThPR1 gene in Tamarix hispida

  • ZHANG Kaimin,WANG Yucheng, YANG Guiyan, GAO Caiqiu*
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Abstract

Pathogenesis-related proteins(PRs)in plants are proteins in plants that induced by biological or abiotic stresses. In this study, the full length cDNA of a PR1(named ThPR1)gene was isolated from seven transcriptome cDNA librarys of T. hispida under NaHCO3 stress. The ThPR1 cDNA was 756 bp, and open reading frame(ORF)was 537 bp, encoding a protein of 178 amino acid residues with the molecular mass of 20.53 ku and theoretical pI of 9.75. The expressions of ThPR1 in T. hispida responding to NaCl, PEG and ABA were further investigated using quantitative RT-PCR. The results showed that ThPR1 was induced in roots and leaves of T.hispida under NaCl stress, but inhibited under PEG and ABA treatments.

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ZHANG Kaimin,WANG Yucheng, YANG Guiyan, GAO Caiqiu. Clone and expression analysis of ThPR1 gene in Tamarix hispida[J]. Journal of Nanjing Forestry University (Natural Sciences Edition). 2013, 37(02): 45-49 https://doi.org/10.3969/j.issn.1000-2006.2013.02.008

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