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中国栗属特有种保守RAPD片段分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2004年04期
Page:
51-53
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Analysis on Conservative DNA Segment for Different Species of Castanea in China by RAPD Marker
Article ID:
1000-2006(2004)04-0051-03
Author(s):
SHEN Yong-bao SHI Ji-sen
National Southern Tree Seed Inspection Center,Nanjing Forestry University,Nanjing 210037,China
Keywords:
RAPD markerCastanea mollissimaC.henyiC.seguiniiIdentificationConservative segment of DNA
Classification number :
Q78
DOI:
10.3969/j.jssn.1000-2006.2004.04.012
Document Code:
A
Abstract:
Identification method of plant species mainly done according to their morphologic characters can not be applied to close species or their parts of tissue.However,development of DNA molecular markers during the past more than 10 years made it possible.This paper studies the application of RAPD molecular marker in distinguishing 3 different species following Castanea mollissima,C.henyi and C.seguinii.The results shown that different special conservative segment of each species was enlarged with primer B07(GTCCACACGG).Respectively three species-specific DNA segments weighted of 2 050 bp,900 bp and 1 500 bp.This method can be used in species identification widely.

References

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Last Update: 1900-01-01