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松枯梢病菌RAPD分子水平遗传距离分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年01期
Page:
13-16
Column:
研究论文
publishdate:
2006-01-20

Article Info:/Info

Title:
RAPD Analysis on Genetic Relationships Among Sphaeropsis sapinea Isolates
Article ID:
1000-2006(2006)01-0013-04
Author(s):
WU Xiao-qin1 XIONG Da-bin2
1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Forestry Administration, Nanjing 210036, China
Keywords:
Sphaeropsis sapinea RAPD Genetic relationship
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2006.01.003
Document Code:
A
Abstract:
Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China, United States, England, South Africa and Chile by using RAPD analysis method. 135 scorable DNA fragments were amplified with twelve random primers by PCR (Polymerase Chain Reaction) technique and the polymorphic score was 96.3%. The genetic dendrogram based on RAPD analysis showed that the all S. sapinea isolates could be clustered into three groups. The isolate CWS41 from Chile was separated genetically as a first group away from other isolates, and isolates F2 and J2 from China were as the second group. The third RAPD group accommodated other rest isolates (including B morphotype CWS43 from United States).

References

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Last Update: 2013-05-20