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西南桦不同种源外植体组织培养技术(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年01期
Page:
27-30
Column:
研究论文
publishdate:
2007-01-20

Article Info:/Info

Title:
Effects of Different Tissue Culture Mediums on Clones Selected from Betula alnoides Provenances
Article ID:
1000-2006(2007)01-0027-04
Author(s):
CHEN Wei1 SHI Jisen1* CHEN Jin-hui1 WANG Chang-shui2 LIU Xi-hua3
1. The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University. Nanjing, 210037 China; 2.Fujian Provincial Forestry Research Center. Nanjing 363600, China; 3.Sanming College Fujian Agriculture and Forestry University, Sanming 365004. China
Keywords:
Betula alnoides Plantlet Tissue culture Provenance
Classification number :
S722;Q813
DOI:
10.3969/j.jssn.1000-2006.2007.01.007
Document Code:
A
Abstract:
Betula alnoides is one of the fast growing and high quality timber production tree spices in southern China, but short of genetic improved planting materials supplying. We presented a series of research results of culture mediums difference to induction, multiplication and rooting ability, by seven clones selected from B. alnoides provenances testing plantation. The result indicated that when choosing the second, and the third buds against shoot tip, as explants, have had a highest sprouting rate cultured on B5 medium with the NAA (0.05mg/L). For multiplication, the best medium improved MS1, and for rooting was 1/3MS with IBA(1.0mg/L)+ABT(0.2mg/L)+NAA(0.2mg/L) medium. Among the different clones, clone M16 was the best responding one to the culture medium, however, such a relationship between tissue culture performance and growth still needs to be carefully investigated.

References

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Last Update: 2013-05-20