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不同生长调节物质对宣木瓜愈伤组织的诱导(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年06期
Page:
125-128
Column:
研究简报
publishdate:
2007-06-20

Article Info:/Info

Title:
Effect of Different Growth Regulators on Callus Induction of Chaenomeles speciosa Nakai
Article ID:
1000-2006(2007)06-0125-04
Author(s):
YE Jian-wei1 YIN Zeng-fang1* HE Zhen-xiang2
1.College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2.School of Life Science Nanjing University, Nanjing 210095, China
Keywords:
Chaenomeles speciosa Nakai Growth regulator Callus tissue Cotyledons Hypocotyls
Classification number :
S718;S661.6
DOI:
10.3969/j.jssn.1000-2006.2007.06.030
Document Code:
A
Abstract:
The effects of different types and concentrations of plant growth regulators on callus tissue induction of cotyledons and hypocotyls of Chaenomeles speciosa Nakai were studied. The results showed that 2,4-D had a better effect on inducing and subculture callus tissue than that of NAA in the experiment of cotyledons. A satisfactory result of callus tissue induction and callus growth was achieved on MS medium supplemented with 1.0mg/L 2,4-D and 0.1mg/L KT. Callus tissue induction rate of hypocotyls was 87%, which was higher than that of cotyledons, i.e., 69%. The proportion and the degree of callus tissue vitrifaction would scale up when the concentration of auxin and BA increased. Best callus tissue of hypocotyls were obtained on MS medium contained 0.1=mg/L 2,4-D and 0.3mg/L BA.

References

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Last Update: 2013-05-20