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无菌松材线虫的获得及培养方法研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2008年03期
Page:
99-102
Column:
研究论文
publishdate:
2008-05-20

Article Info:/Info

Title:
A study of the acquisition of sterilized nematode and growth of the pine wood nematode
Article ID:
1000-2006(2008)03-0099-04
Author(s):
BEN Ai-ling12 HAN Zheng-min1* HAN Xu1 SUN Ping1
1. College of Forest Resources and Environmenl Nanjing Forestry University, Nanjing 210037, China; 2. Department of Life Science, Nanjing Xiaozhuang University, Nanjing 211171, China
Keywords:
Bursaphelenchus xylophilus i Sterilization Antibiotic
Classification number :
S763.3; Q95
DOI:
10.3969/j.jssn.1000-2006.2008.03.024
Document Code:
A
Abstract:
Acquisition of sterilized nematode was the basis of disease mechanism and nematode pathogenic study. Different concentrations of H2O2_ and antibiotics were treated to pine wood nematodes and their eggs. The effective sterilizing process was tested. After an analysis, it was seen that the best way to sterilize the surface of the nematode body was to use higher concentration of antibiotics and lower concentration of H2O2 alternately and repeatedly. The sterilized trematode and the nematode carring bacteria were used in the Botrysls cinerea which grew in the medium of PSA or corn. It was found there were no significant differences in growth and reproduction of the nematode.

References

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Last Update: 2013-05-20