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聚糖对绿色木霉木聚糖酶的吸附规律

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2008年06期
Page:
19-23
Column:
研究论文
publishdate:
2008-11-30

Article Info:/Info

Title:
Adsorption of xylanases from Trichoderma viride to polysaccharides
Article ID:
1000-2006(2008)06-0019-05
Author(s):
JIANG Hua BO Kai-jing
College of Chemical Engineering Nanjing Forestry University, Nanjing 210037, China
Keywords:
Oat-spelt xylan Microcrystalline cellulose Xylanases Adsorption
Classification number :
TQ352
DOI:
10.3969/j.jssn.1000-2006.2008.06.005
Document Code:
A
Abstract:
Adsorption of xylanases from Trichoderma virides to the insoluble oat- spelt xylan and the microerystalline cellulose (MCC) was investigated. It was found that the Langmuir’ s isotherms were suitable for the adsorption of xylanases to the oat-spelt xylan and the MCC, respectively. The adsorption capacities of the oat-spelt xylan and the MCC for the xylanses were 88.9 and 32.4 U/g. The adsorption affinity of xylanases to the oat-spelt xylan was higher than that to MCC. The adsorption of xylanases to the polysaccharides used could be described as the first order kinetic processes. The rate constant of adsorption to oat-spelt xylan was 35% higher than that to MCC. The electrophoresis pattern and Michaelis’ constant showed that the components bounded by the polysaccharides tested were identical. The results suggested that the oat-spelt xylan was a promising candidate adsorbent in the rapid recovery and purification of xylanases.

References

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Last Update: 2013-05-20