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稀土等对霍山石斛组培苗石斛多糖含量的影响

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2008年06期
Page:
39-42
Column:
研究论文
publishdate:
2008-11-30

Article Info:/Info

Title:
Effects of rare earth and so on on polysaccharides content of Dendrobium huoshanense plantlet in vitro
Article ID:
1000-2006(2008)06-0039-04
Author(s):
XIE Yin-feng1XU Li12
1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. Nanjing Institute of Gardening Science, Nanjing 210037, China
Keywords:
Rare earth L - lys Salicylic acid Polysaccharides Dendrobium huoshanense
Classification number :
S718
DOI:
10.3969/j.jssn.1000-2006.2008.06.009
Document Code:
A
Abstract:
The effects of rare earth lanthanum ( 10--40 mg/L) , salicylic acid ( 0.2--5.0 mg/L) and L - lys ( 0.5--12.5 mg/L) on the content of polysaccharides in Dendrobium huoshanense seedling were examined under tissue culture condition. The extraction condition of polysaccharides was determined. The results showed that lanthanum and L - lys had highly significant effects on the polysaccharides content, while salicylic acid had significant effects. The best concentration within each definite concentration range in improving the polysaccharides in D. huoshanense was 20mg/L lanthanum nitrate, 2.5 mg/L L - lys and 0.2 mg/L salicylic acid. The content increased 76.33% , 77. 11% and23.60% respectively compared with the control. The orthogonal test showed that the optimum plan of polysaccharides extraction in D. huoshanense was 100 mL of the content of petroleum ether, 100 mL of 80% using ethanol and 40 mL of the feed. The order of three factors to influence polysaccharides extraction was 80% using ethanol, petroleum ether, material over solvent. The above results could be used as the reference for the development and utilization of D. huoshanense resources.

References

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Last Update: 2013-05-20