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聚苯乙烯微球中荧光共振能量转移的研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年02期
Page:
83-87
Column:
研究论文
publishdate:
2011-03-28

Article Info:/Info

Title:
Study on fluorescence resonance energy transfer in polystyrene microspheres microspheres
Author(s):
WU Weibing1 WANG Mingliang2 JING Yi1 DAI Hongqi1 ZHOU Xiaofan1
1. Jiangsu Provincial Key Lab of Pulp and Paper Science and Technology, Nanjing Forestry University, Nanjing 210037, China; 2. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
Keywords:
polystyrene fluorescent microspheres dispersion polymerization fluorescence resonance energy transfer
Classification number :
Q632,TB34
DOI:
10.3969/j.jssn.1000-2006.2011.02.018
Document Code:
A
Abstract:
A series of fluorescent monodisperse polystyrene microspheres labeled with 1, 8naphthamide dyes were prepared by dispersion copolymerization. Making use of fluorescence resonance energy transfer (FRET), the prepared microspheres showed both the fluorescence of the donor and acceptor dyes under the excitation wavelength of the donor dye. It is found that effective FRET occurs at a relatively low dye concentration and appropriate molar ratio of donor and acceptor. An increase in the dye concentration, which reduces the distance between the donor and acceptor, leads to more effective FRET in microspheres. By changing the dye concentrations, microspheres could show tuned fluorescent signals under a single wavelength excitation. This type of uniform fluorescent microspheres has potential application in bioassays.

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Last Update: 2011-04-13