我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

油茶SRAP-PCR反应体系的建立(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年05期
Page:
112-116
Column:
油茶高效栽培研究专栏
publishdate:
2011-09-30

Article Info:/Info

Title:
Establishment reaction system of the SRAPPCR in Camellia oleifera
Author(s):
WU Yingying1PENG Fangren1*HAO Mingzhuo1CHEN Longsheng*
1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2. Hunan Forestry Academy, Changsha 410004, China
Keywords:
Camellia oleifera SRAP reaction system orthogonal design
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2011.05.025
Document Code:
A
Abstract:
In this paper, orthogonal design to SRAPPCR system for Camellia oleifera cultivar Dabieshan 2 and Ganxing 46 was conducted with the primers combination of Me5 and Em8. Orthogonal designdirect analysis and variance analysis were applied to optimize SRAPPCR amplification system in 4 factors such as Taq DNA polymerase, Mg2+, dNTP and primer. Template DNA was also analyzed by the single element test. The results indicated that an optimal reaction system (20 μL)of SRAPPCR system was established: 120 μmol/min Taq DNA polymerase 125 mmol/L Mg2+, 0.15 mmol/L dNTP, 0.60 μmol/L primer, 60 ng template DNA and 2 μL 10 buffer(Mg2+ free). And, the order of each factor levels affected on the result of SRAPPCR was dNTP, Mg2+, Taq DNA polymerase and primer.

References

[1]庄瑞林.中国油茶[M].北京:中国林业出版社,2008.
[2]陈永忠,张智俊,谭晓风.油茶优良无性系的RAPD分子鉴别[J].中南林学院学报,2005,25(4):40-45.
[3]黄永芳,陈锡林,庄雷影,等.油茶种质资源遗传多样性分析[J].林业科学,2006,42(4):38-43.
[4]温强,雷小林,叶金山,等.油茶高产无性系的ISSR分子鉴别[J].中南林业科技大学学报,2008,28(1):39-43.
[5]林萍,姚小华,王开良,等.油茶长林系列优良无性系的SRAP分子鉴别及遗传分析[J].农业生物技术学报,2010,18(2):272-279.
[6]曾柏全,邓子牛,杨迎花,等.湖南宽皮柑橘SRAP的反应体系[J].中南林业科技大学学报,2008,28(6):71-74.
[7]祝全东,张党权,李晓云,等.油茶SRAP标记的PCR体系建立与优化[J].中南林业科技大学学报,2010,30(3):57-62.
[8]谭碧玥,王源秀,徐立安.分子标记SRAP及其在林木研究中的应用[J].世界林业研究,2009,22(5):45-50.
[9]王燕青.利用SRAP标记构建菏泽牡丹优良品种指纹图谱[D].南京:南京林业大学,2008.
[10]郭大勇,徐育海,张靖国,等.湖北海棠种内遗传变异的SRAP分析[J].果树学报,2009,26(6):886-890.
[11]Wang G, Pan J, Li X, et al. Construction of a cucumber genetic linkage map with SRAP markers and location of the genes for lateral branch traits[J]. Science in China Series C:Life Science, 2005, 48(3): 213-220.
[12]Li G, Gao M, Yang B, et al. Gene for gene alignment between the Brassica and Arobidopsis genomes by direct transcriptome mapping[J]. Theor Appl Genet, 2003, 107(1): 168-180.
[13]杨向辉,黄建峰,傅嘉欣,等.黄皮SRAP反应体系优化正交实验研究[J].亚热带植物科学,2009,38(4):27-30.
[14]司鹏,戴洪义,薛华柏,等.苹果SRAP-PCR反应体系的建立[J].果树学报,2010,27(2):168-173.
[15]Budak H, Shearman R C, Parmaksiz I, et al. Molecular characterization of buffalograss germplasm using sequence related amplified polymorphism markers [J]. Theor Apple Genet, 2004, 108(2): 328-334.

Last Update: 2011-09-30