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麝香百合及东方百合品种核型分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年01期
Page:
67-70
Column:
研究论文
publishdate:
2013-01-15

Article Info:/Info

Title:
The karyotype analysis of longiflorum lily and oriental lily
Author(s):
FANG Lei JIANG Shan XI Mengli* SHI Jisen FU Wei YANG Bin
Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
Keywords:
longiflorum lily oriental lily karyotype analysis
Classification number :
S722; Q948
DOI:
10.3969/j.issn.1000-2006.2013.01.011
Document Code:
A
Abstract:
Plantlet root tips of longiflorum hyhrids ‘White Fox’ and ‘Lei Shan’, oriental hybrids ‘Crystal Blanca’ and ‘Constansa’ were used as materials to analyze karyotype. The root tips were pretreated with cyclohexamide then fixed in Carnoy’s solution and cell wall was digested with cellulase and pectinase. Finally, spreading method was used for preparing slides. The karyotype analysis result indicated that the karyotype of ‘White Fox’ was 3A, that of others were 3B. The karyotype formula was 2n=2x=24=4m+4st(2SAT)+16t for ‘White Fox’, 2n=2x=24=2m+2sm+10st(2SAT)+10t for ‘Lei Shan’, 2n=2x=24=4m+10st+10t for ‘Crystal Blanca’, 2n=2x=24=4m(1SAT)+10st+10t for ‘Constansa’. Karyotype asymmetry coefficient were 83.11%, 82.78%, 79.75%, 81.22%, respectively.

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Last Update: 2013-01-15