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橡胶树糖代谢调控基因HbF2KP的克隆及表达(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年04期
Page:
23-28
Column:
研究论文
publishdate:
2014-07-01

Article Info:/Info

Title:
Molecular cloning and expression analysis of HbF2KP from Hevea brasiliensis
Article ID:
1000-2006(2014)04-0023-06
Author(s):
LONG Xiangyu1 HE Bin2 CAO Bing2 LIANG Qifu2 FANG Yongjun1 YANG Jianghua1 TANG Chaorong1*
1. Rubber Research Institute, CATAS, Danzhou 571737, China;
2. College of Agriculture, Hainan University, Haikou 570228, China
Keywords:
Hevea brasiliensis HbF2KP gene cloning gene expression bioinformatics analysis
Classification number :
Q78; S794.1
DOI:
10.3969/j.issn.1000-2006.2014.04.005
Document Code:
A
Abstract:
The cDNA sequence of HbF2KP was isolated and analyzed in Hevea brasiliensis for the first time. HbF2KP contained a 2 244 bp long ORF that predicted a protein of 747 amino acids. Bioinformatics analyses were performed on HbF2KP protein. HbF2KP had highly conserved kinase and phosphatase domains of fructose-6-phosphate,2-kinase/fructose-2,6-bisphosphatase. Due to the characteristics of high hydrophilicity, no signal peptide and transmembrane domains, it was inferred that HbF2KP function mainly in the cytoplasm. Real-time PCR analysis revealed that the expression of HbF2KP was constitutive in both photosynthetic and non-photosynthetic tissues. HbF2KP expressions were affected in the latex by tapping, wounding and tapping panel dryness, which suggests that HbF2KP play an important role in the regulation of sugar metabolism, especially in rubber biosynthesis and stress response to tapping panel dryness. In addition, the expression of HbF2KP was induced by some hormones, such as ET, 2,4-D, ABA, SA and GA.

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Last Update: 2014-07-31