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青杄PwWrbA基因克隆及表达分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年04期
Page:
34-38
Column:
研究论文
publishdate:
2014-07-01

Article Info:/Info

Title:
Cloning and expression analysis of WrbA gene from Picea wilsonii
Article ID:
1000-2006(2014)04-0034-05
Author(s):
ZHANG TongZHOU YanniLI ChangjiangZHANG Lingyun*
Key Laboratory of Forest Silviculture and Conservation of the Ministry of Education,Beijing Forestry University,Beijing 100083,China
Keywords:
Picea wilsonii WrbA bioinformatics tissue expression stress response
Classification number :
Q78; S792
DOI:
10.3969/j.issn.1000-2006.2014.04.007
Document Code:
A
Abstract:
The tryptophan(W)repressor-binding protein(WrbA)is an important antioxidant enzyme which has been shown to play roles in a variety of adversities. In this study,PwWrbA cDNA was cloned by RACE-PCR method. Bioinformatics analysis showed that the full length of PwWrbA cDNA was 1 045 bp, including the ORF 651 bp, encoding 203 amino acids. The theoretical molecular weight was 21.80 ku and value of pI was 6.43.The ScanProsite online software showed that the N terminal 11-15 aa and the C terminal 112-165 aa were supposed to be the FMN binding sites. RT-qPCR and semi-quantitative RT-PCR analysis showed PwWrbA expressed in various tissues, while the highest expression level was in pollen. Meanwhile, the expression of PwWrbA increased gradually after stress treatment with NaCl and ABA.In contrast to NaCl and ABA treatment, the expression of PwWrbA decreased in early stage and increased at 6 h after H2O2 treatment. Our results suggested that PwWrbA was a new WrbA gene and played roles during different abiotic stresses.

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Last Update: 2014-07-31