我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

拟松材线虫不同致病力虫株体内细菌的种类差异(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年04期
Page:
63-68
Column:
研究论文
publishdate:
2015-07-20

Article Info:/Info

Title:
Endobacteria from different virulence Bursaphelenchus mucronatus
Article ID:
1000-2006(2015)04-0063-06
Author(s):
YUAN Weimin12 XIANG Yang12 WU Xiaoqin12*
1.Co-Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2.Jiangsu Key Laboratory of Pest Prevention and Control,College of Forestry, Nanjing Forestry University,Nanjing 210037,China
Keywords:
Bursaphelenchus mucronatus pathogenic Pinus thunbergii endobacteria
Classification number :
S763.16
DOI:
10.3969/j.issn.1000-2006.2015.04.011
Document Code:
A
Abstract:
Bursaphelenchus mucronatus is one of plant parasitic nematodes and similar to the causative agent of pine wilt disease B. xylophilus in morphology. To understand the interaction between B. mucronatus and endobacteria, ten strains of B. mucronatus from seven provinces were inoculated to 2-year old Pinus thunbergii to evaluate their virulence. Besides, endobacteria of B. mucronatus were also isolated and analyzed. Pathogenic measurement showed that six strains of B. mucronatus were pathogenic to P.thunbergii, but the other four strains had no pathogenicity. Seventeen strains of endobacteria were isolated from 9 of 10 B. mucronatus strains. Physiological and biochemical characteristics, biolog methods combined with molecular revealed that seventeen strains of endobacteria belonged to Stenotrophomonas maltophilia, Ewingella americana, Pseudomonas fluorescens, Agrobacterium tumefaciens,Luteibacter anthropi, Microbacterium oxydans, Agreia pratensis, Leifsonia naganoensis and Achromobacter xylosoxidans ssp. xylosoxidans. Further analysis showed that species and population of endobacteria strains from virulent B. mucronatus strains were more abundant than that from non-virulent strains. S. maltophilia were usually isolated from virulent B. mucronatus strains, while endobacteria M. oxydans were mainly isolated from avirulent strains.

References

[1] 杨宝君. 松材线虫病 [M]. 北京:中国林业出版社, 2003.Yang B J. Pine wilt disease[M]. Beijing: Chinese Forestry Publishing House, 2003.
[2] Mota M M, Braasch H, Bravo M A, et al. First report of Bursaphelenchus xylophilus in Portugal and in Europe [J]. Nematology, 1999, 1(7): 727-734.
[3] Fonseca L, Cardoso J, Lopes A, et al. The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island [J]. Helminthologia, 2012, 49(2): 96-103.
[4] Abelleira A, Picoaga A, Mansilla A, et al. Detection of Bursaphelenchus xylophilus, causal agent of pine wilt disease on Pinus pinaster in Northwestern Spain [J]. Florida Entomologist, 2013, 96(3): 770-780.
[5] Cheng X Y, Xie P Z, Cheng F X, et al. Competitive displacement of the native species Bursaphelenchus mucronatus by an alien species Bursaphelenchus xylophilus(Nematoda: Aphelenchida: Aphelenchoididae): a case of successful invasion [J]. Biological Invasions, 2009, 11(2): 205-213.
[6] Rutherford T, Riga E, Webster J. Temperature-mediated behavioral relationships in Bursaphelenchus xylophilus, B. mucronatus, and their hybrids [J]. Journal of Nematology, 1992, 24(1): 40.
[7] Jikumaru S, Togashi K. Boarding abilities of Bursaphelenchus mucronatus and B. xylophilus (Nematoda: Aphelenchoididae)on Monochamus alternatus(Coleoptera: Cerambycidae)[J]. Nematology, 2003, 5(6): 843-849.
[8] Mamiya Y, Enda N. Bursaphelenchus mucronatus n. sp.(Nematoda: Aphelenchoididae)from pine wood and its biology and pathogenicity to pine trees [J]. Nematologica, 1979, 25(3):353-361.
[9] Tomminen J. Pathogenicity studies with Bursaphelenchus mucronatus in Scots pine in Finland [J]. European Journal of Forest Pathology, 1993, 23(4): 236-243.
[10] 程瑚瑞, 林茂松, 钱汝驹. 拟松材线虫(Bursaphelenchus mucronatus)的形态诊断和致病性研究 [J]. 南京农业大学学报, 1986,5(2):55-61.Cheng H R, Lin M S, Qian R J. A study on the morohological diagnosis and the pathogenicity of Bursaphelenchus mucronatus[J]. Journal of Nanjing Agricultural University, 1986,5(2):55-61.
[11] Braasch H, Caroppo S, Ambrogioni L, et al. Pathogenicity of various Bursaphelenchus species to pines and implications to European forests[C]//Kazuyoshi F, Katsumi T, Takefumi I. Sustainability of pine forests in relation to pine wilt and decline. Proceedings of International Symposium. Tokyo: Shokado, 1999.
[12] Bakke A, Anderson R, Kvamme T. Pathogenicity of the nematodes Bursaphelenchus xylophilus and B. mucronatus to Pinus sylvestris seedlings: a greenhouse test [J]. Scandinavian Journal of Forest Research, 1991, 6(1-4): 407-12.
[13] Kanzaki N, Futai K. Is Bursaphelenchus mucronatus a weak pathogen to the Japanese red pine? [J]. Nematology, 2006, 8(4): 485-489.
[14] Kawazu K, Kaneko N. Asepsis of the pine wood nematode isolate OKD-3 causes it to lose its pathogenicity [J]. Japanese Journal of Nematology, 1997, 27(2): 76-80.
[15] Kawazu K, Yamashita H, Kobayashi A,et al. Isolation of pine-wilting bacteria accompanying pine(Pinus)wood nematode, Bursaphelenchus xylophilus, and their toxic metabolites [J]. Scientific Reports of the Faculty of Agriculture-Okayama University, 1998, 87:1-7.
[16] 赵博光, 刘玉涛, 林峰. 松材线虫与其携带细菌之间的相互影响 [J]. 南京林业大学学报: 自然科学版, 2005, 29(3): 1-4.Zhao B G, Liu Y T, Lin F. Mutual influences between Bursaphelenchus xylophilus and bacteria carries[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2005, 29(3): 1-4.
[17] 谈家金, 向红琼, 冯志新. 松材线虫伴生细菌的分离鉴定及其致病性 [J]. 林业科技开发, 2008, 22(2): 23-6.Tan J J, Xiang H Q, Feng Z X. A preliminary study on isolation,identification and pathogenicity of the bacterium accompanying Bursaphelenchus xylophilus [J]. China Forestry Science and Technology, 2008, 22(2): 23-6.
[18] Wu X Q, Yuan W M, Tian X J, et al. Specific and functional diversity of endophytic bacteria from pine wood nematode Bursaphelenchus xylophilus with different virulence [J]. International Journal of Biological Sciences, 2013, 9(1): 34-44.
[19] 袁为敏, 吴小芹, 叶建仁,等. 松材线虫和拟松材线虫体内细菌的透射电镜观察及分离鉴定[J]. 微生物学报, 2011, 51(8): 1071-1077.Yuan W M, Wu X Q, Ye J R, et al. Observation by transmission electron microscope andidentification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus[J]. Acta Microbiologica Sinica, 2011, 51(8): 1071-1077.
[20] Dong X Z, Cai M Y. Identification system manual of common bacteria[J]. Beijing: Science Press, 2001.
[21] 张治宇,张克云,林茂松,等. 不同松材线虫群体对黑松的致病性测定 [J]. 南京农业大学学报, 2002, 25(2): 43-46.Zhang Z Y, Zhang K Y, Lin M S, et al. phthogenticity determination of Bursanhelenchus xylophilus to pine thunbergii[J]. Journal of Nanjing Agricultural University,2002,25(2):43-46.
[22] 张兴华. 拟松材线虫体表微生物及其致病作用研究 [D]. 成都:四川农业大学, 2009.Zhang X H. Study on the microorganism carried by Bursaphelenehus mucronatus and their pathogenicity [D]. Chengdu: Sichuan Agricultural University, 2009.
[23] Delalibera J R I, Vasanthakumar A, Burwitz B J, et al. Composition of the bacterial community in the gut of the pine engraver, Ips pini(Say)(Coleoptera)colonizing red pine [J]. Symbiosis(Rehovot), 2007, 43(2): 97-104.
[24] Zhang X, Yuen G. Effects of culture fluids and preinduction of chitinase production on biocontrol of bipolaris leaf spot by Stenotrophomonas maltophilia C3[J]. Biological Control, 2000, 18(3):277-286.
[25] Hollak C, Van weely S, Van oers M, et al. Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease [J]. Journal of Clinical Investigation, 1994, 93(3): 1288.
[26] H?fte M, De Vos P. Plant pathogenic Pseudomonas species[C]//Plant-associated bacteria. Netherlands:Springer, 2006: 507-533.
[27] Torres A R, Araujo W L, Cursino L, et al. Diversity of endophytic enterobacteria associated with different host plants [J]. The Journal of Microbiology, 2008, 46(4): 373-379.

Last Update: 2015-07-31