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拟松材线虫组织蛋白酶基因Bmcath1的序列分析 与转录水平检测(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年06期
Page:
12-16
Column:
专题报道
publishdate:
2015-11-30

Article Info:/Info

Title:
Bioinformatic and expression analysis of a cathepsin gene Bmcath1 in Bursaphelechus mucronatus
Article ID:
1000-2006(2015)06-0012-05
Author(s):
PAN Yanyi12 HUANG Lin12 WU Xiaoqin12*
1.Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2.Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Bursaphelenchus mucronatus cathepsin phylogenetic tree gene expression profiling
Classification number :
S763.16
DOI:
10.3969/j.issn.1000-2006.2015.06.003
Document Code:
A
Abstract:
Cathepsin gene Bmcath1 of Bursaphelenchus mucronatus is differentially expressed between B. xylophilus and B. mucronatus. In order to understand its biological function, bioinformational analysis was conducted using software BioEdit, SignalP 4.1, BLASTP and MEGA 4. 0. The result showed that the full-length cDNA of Bmcath1 was 1 294 bp long, which included an ORF of 1 158 bp. The deduced amino sequence of CATH1 contained 386 amino acids with molecular mass of 42.38 ku and pI of 8.52. Protein CATH1 contained a Peptidase-C1A conserved domain. The amino sequences of cathepsins varied between different species. With the method of real-time PCR, gene expression profiling showed that Bmcath1 expressed much more in B. mucronatus separated from Pinus thunbergii seedlings comparing with expression level of Bmcath1 in monoxenic culture nematodes. The result suggested that function of Bmcath1 may be related to infection process of B. mucronatus.

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Last Update: 2015-11-30