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蜡梅SAMT基因遗传转化及其功能分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年06期
Page:
29-34
Column:
研究论文
publishdate:
2015-11-30

Article Info:/Info

Title:
Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox
Article ID:
1000-2006(2015)06-0029-06
Author(s):
PANG Hongdong1 2 XIANG Lin3 ZHAO Kaige1 LI Xiang1YANG Nan1 CHEN Longqing1*
1.Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China;
2. Hubei Academy of Forestry, Wuhan 430075, China;
3 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China
Keywords:
Chimonanthus praecox CpSAMT genetic transformation Petunia hybrida floral scent metabolic puthway GC-MS
Classification number :
Q78; S685
DOI:
10.3969/j.issn.1000-2006.2015.06.006
Document Code:
A
Abstract:
In order to investigate the biological function of the Chimonanthus praecox SAMT gene, here we reported that the plant binary expression vector pBI121-CpSAMT was constructed and then the CpSAMT gene was introduced into petunia through Agrobacterium tumefaciens mediated transformation. PCR and RT-PCR was used to test the transgenic plants, and HS-SPME-GC-MS was employed to analyze the components of flower flavor. Results showed that nine independent transgenic lines obtained were positive. Further analysis showed that transgenic plants were indistinguishable from untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering time. The analysis of HS-SPME-GC-MS showed that the content of benzaldehyde, phenylethanol, citronellol, citronellyl acetate and phenethyl acetate in flowers of transgenic CpSAMT plants was increased. However, the contents of methyl salicylate and methyl benzoate had not been significantly changed comparing with non-transgenic controls.

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Last Update: 2015-11-30