我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

凝结芽孢杆菌中β-半乳糖苷酶基因的克隆及表达(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年06期
Page:
35-39
Column:
研究论文
publishdate:
2015-11-30

Article Info:/Info

Title:
Molecular cloning and expression of a novel β-galactosidase from Bacillus coagulans
Article ID:
1000-2006(2015)06-0035-05
Author(s):
ZHENG Zhaojuan XU Ying SHI Lei OUYANG Jia*
College of Chemical Engineering, Nanjing Forestry University, Jiangsu Key Lab of Biomass-Based Green Fuels and Chemicals, Nanjing 210037, China
Keywords:
Bacillus coagulans β-galactosidase expression and purification lactose hydrolysis
Classification number :
Q815; TQ93
DOI:
10.3969/j.issn.1000-2006.2015.06.007
Document Code:
A
Abstract:
A novel β-galactosidase gene was cloned from Bacillus coagulans NL01 which had ability to hydrolyze lactose into glucose and galactose in this study. The length of the β-galactosidase gene was 1 998 bp, and its coding sequence showed very low identity with other reported β-galactosidase. The gene was cloned into pETDuet-1 and expressed in Escherichia coli BL21(DE3). The crude enzyme activity was 119.0 μmol/(min·mg), and the purified enzyme activity was 666.4 μmol/(min·mg)after Ni-NTA purification. It was indicated from the analysis results of the hydrolysis product obtained by the purified β-galactosidase that this novel β-galactosidase presented high activity toward lactose conversion into glucose and galactose.

References

[1] Mlichova Z, Rosenberg M.Current trends of β-galactosidase application in food technology[J]. Journal of Food and Nutrition Research, 2006, 45(2): 47-54.
[2] Park A R, Oh D K. Galacto-oligosaccharide production using microbial β-galactosidase: current state and perspectives[J]. Applied Microbiology and Biotechnology, 2010, 85(5): 1279-1286.
[3] Ansari S A, Satar R. Recombinant β-galactosidases-Past, present and future: A mini review[J]. Journal of Molecular Catalysis B: Enzymatic, 2012, 81: 1-6.
[4] Panesar P S, Panesar R, Singh R S, et al. Microbial production, immobilization and applications of β-D-galactosidase[J]. Journal of Chemical Technology and Biotechnology, 2006, 81(4): 530-543.
[5] 董艺凝, 陈海琴, 刘小鸣, 等. 耐热β-半乳糖苷酶的研究进展[J]. 食品工业科技, 2012, 33(1): 384-387. Dong Y N, Chen H Q, Liu X M, et al. Research progress in thermostable β-galactosidases[J]. Science and Technology of Food Industry, 2012, 33(1): 384-387.
[6] Ouyang J, Ma R, Zheng Z J, et al. Open fermentative production of L-lactic acid by Bacillus sp. strain NL01 using lignocellulosic hydrolyzates as low-cost raw material[J]. Bioresource Technology, 2013, 135: 475-480.
[7] 张刚. 乳酸细菌-基础、技术和应用[M]. 北京: 化学工业出版社, 2007. Zhang G. Lactic acid bacteria-basic, technology and application[M]. Beijing: Chemical Industry Press, 2007.
[8] 凌代文, 东秀珠. 乳酸细菌分类鉴定及实验方法[M]. 北京: 中国轻工业出版社, 1999. Ling D W, Dong X Z. Classification and identification of lactic acid bacteria and experimental method[M]. Beijing: China Light Industry Press, 1999.
[9] Sun X J, Duan X G, Wu D, et al.Characterization of Sulfolobus solfataricus β-galactosidase mutant F441Y expressed in Pichia pastoris[J]. Journal of the Science of Food and Agriculture, 2014, 94: 1359-1365.
[10] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72: 248-254.
[11] Li Z Y, Xiao M, Lu L L, et al. Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells[J]. Process Biochemistry, 2008, 43(8): 896-899.
[12] Henrissat B, Bairoch A. A classification of glycosyl hydrolases based on amino acid sequence similarities[J]. Biochem J, 1991, 280: 309-316.
[13] Henrissat Bairoch A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities[J]. Biochemical Journal, 1993, 293: 781-788.
[14] Nguyen T T, Nguyen H A, Arreola S L, et al. Homodimeric β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus DSM 20081: expression in Lactobacillus plantarum and biochemical characterization[J]. Journal of Agricultural and Food Chemistry, 2012,60(7): 1713-1721.
[15] Lu L L, Xiao M, Li Z Y, et al. A novel transglycosylating β-galactosidase from Enterobacter cloacae B5[J]. Process Biochemistry, 2009, 44(2): 232-236.
[16] Li Y M, Lu L L, Wang H M, et al. Cell surface engineering of a β-galactosidase for galactooligosaccharide synthesis[J]. Applied and Environment Microbiology, 2009, 75(18): 5938-5942.
[17] Li Y M, Wang H M, Lu L L, et al. Purification and characterization of a novel β-galactosidase with transglycosylation activity from Bacillus megaterium 2-37-4-1[J]. Applied Biochemistry and Biotechnology, 2009, 158(1): 192-199.
[18] Wang K, Li G, Yu S Q, et al. A novel metagenome-derived β-galactosidase: gene cloning, overexpression, purification and characterization[J]. Applied Microbiology and Biotechnology, 2010, 88(1): 155-165.
[19] Zhang X, Li H, Li C J, et al. Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin[J]. BMC Microbiology, 2013, 13: 237.
[20] 陆文伟, 孔文涛, 孔健, 等. 发酵乳杆菌β-半乳糖苷酶转糖基活性研究[J].山东大学学报:理学版, 2008, 43(7): 83-87. Lu W W, Kong W T, Kong J, et al. Transgalactosylation activity of beta-galactosidase produced by Lactobacillus fermentum K4[J]. Journal of Shandong University:Natural Science,2008,43(7):83-87.

Last Update: 2015-11-30