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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:

2019年05期

Page:

59-66

Column:

研究论文

publishdate:

2019-09-20

Article Info:/Info

Title:

SSR loci associated with population adaptation in Eucalyptus cloeziana

Article ID:

1000-2006(2019)05-0059-08

Author(s):

WANG Li1; LI Changrong2; LI Fagen1; ZHOU Changpin1; WENG Qijie1;  L? Jiabin1; CHEN Jianbo2; CHEN Jiancheng3; GAN Siming 1; 2?

(1. Key Laboratory of State Forestry Administration on Tropical Forestry Research, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou 510520, China; 2. Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation & Key Laboratory of Central South Fast-growing Timber Cultivation of Forestry Ministry of China, Guangxi Forestry Research Institute, Nanning 530002, China; 3. Yulin Forestry Research Institute, Rongxian 537501, China)

Keywords:

Eucalyptus cloeziana;  SSR marker;  climatic factor;  positive selection;  population adaptation;  isolation by distance (IBD)

Classification number :

S718. 51

DOI:

10.3969/j.issn.1000-2006.201808044

Document Code:

A

Abstract:

【Objective】 Genomic loci that are significantly associated with population adaptation were detected in Eucalyptus cloeziana F. Muell. populations to obtain useful molecular information for germplasm conservation and utilization.【Method】 Seven northern and southern populations of E. cloeziana were analyzed using 84 simple sequence repeats (SSR) markers [29 genomic SSRs and 55 expressed sequence tag (EST) derived SSRs]. Isolation by distance (IBD) among populations was determined using Mantel test. Outlier loci of F-statistics of between-population differentiation (Fst) and their significant allelic associations with habitat climatic variables were investigated for all the SSR loci, with further functional annotation of the significantly associated loci against the NCBI non-redundant protein database. 【Result】 IBD was revealed between the northern and southern populations of E. cloeziana, and the clustering analysis based on 19 climatic variables also resulted in division of the northern and southern populations into independent groups, suggesting the climatic effect on driving the population divergence. A total of 39 Fst outliers (46. 4%) were identified as selective loci. Specifically, the software LOSITAN detected 12 positive and 17 balancing selection loci. Six alleles from five outlier loci were identified with spatial analysis methods, each associated significantly with one or more climatic factors(P < 0. 001) and showed dramatic difference in allelic frequency between the northern and southern populations.Among the six significant alleles, Embra6-118 bp was associated with the minimum temperature of the coldest month (Tmcm), with the locus Embra6 functionally annotated as basic helix-loop-helix (bHLH) transcript factor bHLH155.The allele Embra20-121 bp was associated with the precipitation of the warmest quarter ( Pwq ), with Embra20 functionally annotated as sucrose transporters. EUCeSSR676-168 bp was associated with Tmcm, Pwq, the mean annual temperature (Tma) and the minimum temperature of the warmest month (Tmwm), with the locus functionally annotated as photosystem II stability/ assembly factor HCF136. However, the two other significant markers EUCeSSR298 and EUCeSSR1009 were of unknown function. 【Conclusion】 The divergence between the northern and southern populations of E.cloeziana was strongly related with historical climate, and there might be glacial refugia in the northern and southern zones during the Quaternary. The marked difference in the frequency of climate-associated SSR alleles between the northern and southern populations provides molecular evidence for positive selection oriented climatic adaptation in E.cloeziana.

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Memo

Memo:

收稿日期:2018-11-09　　　　修回日期:2018-12-19 　基金项目:广西创新驱动发展专项(桂科AA17204087-3);“广西主要用材林资源高效培育与利用人才小高地”专项(桂财社函 [2018]112 号)。　第一作者:王莉(wangli3hao@ 126. com),实验工作和数据分析的具体执行人,完成论文初稿的写作与修改,ORCID (0000-0002- 3206-7854);李昌荣(andyharry@ 126. com), 负责采样和实验工作及数据分析。?通信作者:甘四明(siminggan@ caf. ac. cn),研究员,ORCID (0000-0001-6677-9860)。　引文格式:王莉,李昌,李发根,等. 大花序桉群体适应性相关的SSR 位点[J]. 南京林业大学学报(自然科学版),2019,43(5): 59-66.

Common functions

Last Update: 2019-10-08