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|Table of Contents|

拟松材线虫个体发育研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年06期
Page:
11-17
Column:
专题报道
publishdate:
2019-11-25

Article Info:/Info

Title:
Ontogenesis of pine parasitic nematode Bursaphelenchus mucronatus (Nematoda: Aphelenchoididae)
Article ID:
1000-2006(2019)06-0011-07
Author(s):
SHANG Zheng ZHOU Lifeng FENG Yueyao LIU Feiying CHEN Fengmao*
(Co-Innovation Center of the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University,Nanjing 210037,China)
Keywords:
Bursaphelenchus mucronatus spawning embryo ontogenesis
Classification number :
S763.1
DOI:
10.3969/j.issn.1000-2006.201902026
Document Code:
-
Abstract:
【Objective】Explore the reproductive capacity, key stages and morphological changes of eggs in the embryonic development of Bursaphelenchus mucronatus. In addition, time required for the embryonic development and completion of the entire lifecycle were studied to provide a scientific basis for further study of its growth and development.【Method】A total of 180 female adult nematodes in three groups were selected to observe their behavior of egg-laying process at 25 ℃. The cumulative production of the eggs of each group of nematodes was counted every 2 hours until the number of eggs became constant. To observe the entire embryonic development process, the pregnant female nematodes in petri dishes were placed under a Zeiss stereo microscope, and the process of embryonic development was photographed. The time required from egg laying to each key stages of the B. mucronatus embryo was recorded at 25 ℃. Approximately 200 eggs of B. mucronatus were selected and their total hatching rates were calculated every 4 h after 24 hours of development at 25 ℃ until the number of hatchings no longer increased, in 3 sets of replicates. The newly hatched J2 were inoculated onto PDA plates containing the well-grown Botrytis cinerea. Then, on the day 1, 2 and 3 after inoculation of the J2, the nematodes were collected using the Berman funnel method, and the proportions of each age line were calculated to learn the time for nematodes to complete their entire lifecycle.【Result】① B. mucronatus has a good egg-laying ability. The total number of eggs increases rapidly within the first 10 hours. After 16 hours, the numbers of eggs laid gradually reached constant, with an average of 12 eggs/nematode in 28 hours. ② The development process of B. mucronatus embryos mainly comprised of the following key stages: single-cell stage, 2-cell stage, 3-cell stage, 4-cell stage, 5-cell stage, 8-cell stage, 16-cell stage, blastocyst stage, Lima bean stage, tadpole stage, worm stage, first-stage juveniles(J1)and the stage of second-generation juveniles(J2). ③ In the early stages of embryonic development, two types of depressions, i.e., one-half and one-third, occurred. In addition, two different developmental patterns were observed during the 2-cell stage to 5-cell stage. The first one is that two cells do not move but split directly into three cells; the other one is that the cells move to a form of triangle. It was revealed by observing the development of the first cleavage of 30 eggs and the development of the 2-cell stage of 100 eggs that these development patterns are common. ④ At 25 ℃, the cumulative hatching rate of B. mucronatus eggs increased with time, reaching 93.31% after 32 h, and then gradually leveled off. ⑤ The time required for the entire embryogenesis of B. mucronatus eggs from single cell to each of the key stages at 25 ℃ took about 28 hours. The J2 of B. mucronatus were able to generate new J2 after 3 days of inoculation with B. cinerea, and the entire life cycle of B. mucronatus completed in just 3 days.【Conclusion】 The entire embryogenesis process of B. mucronatus eggs from single cell to hatching took about 28 hours at 25 ℃. B. mucronatus can complete its entire lifecycle in just 3 days. The 16 hours are the best time to collect a large number of spawning eggs, and the 36 hours are the best time to collect J2. Further studies are worthwhile on the significance of each of the development stages in the early embryonic development of B. mucronatus.

References

[1] MAMIYA Y, ENDA N. Bursaphelenchus mucronatus n. sp.(Nematoda: Aphelenchoididae)from pine wood and its biology and pathogenicity to pine trees[J]. Nematologica,1979,3(3):353-361. DOI:10.1163/187529279x00091.
[2] AKBULUT S, YüKSEL B, SERIN M, et al. Comparison of pathogenic potential of Bursaphelenchus species on conifer seedlings between greenhouse and outdoor conditions[J]. Phytoparasitica, 2015, 43(2): 209-214. DOI:10.1007/s12600-014-0433-2.
[3] ZHOU L F, CHEN F M, YE J R, et al. Selection of reliable reference genes for RT-qPCR analysis of Bursaphelenchus mucronatus gene expression from different habitats and developmental stages[J]. Frontiers in Genetics, 2018, 9: 269. DOI:10.3389/fgene.2018.00269.https://doi.org/10.3389/fgene.2018.00269.
[4] 陈凤毛,史延梅,王姝颖, 等. 不同株系拟松材线虫对黄山松和黑松苗木的致病性[J]. 林业科学, 2010, 46(12): 86-90. DOI:10.11707/j.1001-7488.20101214. CHEN F M, SHI Y M, WANG S Y, et al. Pathogenicity of different isolates of Bursaphelenchus mucronatus to Pinus taiwanensis and P. thunbergii seedlings[J]. Scientia Silvae Sinicae, 2010, 46(12): 86-90.
[5] ZHOU L F, CHEN F M, WANG J C, et al. Virulence of Bursaphelenchus mucronatus to pine seedlings and trees under field cownditions[J]. Forest Pathology, 2016, 46(6): 643-651. DOI:10.1111/efp.12285.
[6] BETTRIZ S N, EVA G O, ZHELEVA A, et al. The embryonic cell lineage of Caenorhabditis elegans: a modern hieroglyph[J]. BioEssays, 2014, 37(3): 237-239. DOI:10.1002/bies.201400202.
[7] UWE D, EINHARD S, THOMAS C, et al. Cell lineages of the embryo of the nematode Caenorhabditis elegans[J]. Developmental Biology,1975,75(1):376-380. DOI:10.1073/pnas.75.1.376.
[8] SCHNABEL R, WEIGNER C, HUTTER H, et al. Mex-1 and the general partitioning of cell fate in the early C. elegans embryo[J]. Mechanisms of Development, 1996, 54(2): 133-147. DOI:10.1016/0925-4773(95)00466-1.
[9] 应晨希,吴小芹. 松材线虫与拟松材线虫种间杂交特性研究[J]. 南京林业大学学报(自然科学版), 2014, 38(6): 48-54. DOI:10.3969/j.issn.1000-2006.2014.06.010. YING C X, WU X Q. Characteristics of hybridization between Bursaphelenchus xylophilus and B. mucronatus[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2014, 38(6): 48-54.
[10] VIGLIERCHIO D R, SCHMITT R V.On the methodology of nematode extraction from field samples: Baermann funnel modifications[J]. Journal of Nematology,1983,15(3):438-444.
[11] 朱丽华,应晨希,叶建仁. 松材线虫近交系的培育[J]. 林业科学,2011,47(12):92-96. DOI:10.11707/j.1001-7488.20111213. ZHU L H, YING C X, YE J R. Inbreeding of the pine wood nematode,Bursaphelenchus xylophilus[J]. Scientia Silvae Sinicae, 2011, 47(12): 92-96.
[12] HASEGAWA K, FUTAI K, MIWA S, et al. Early embryogenesis of the pinewood nematode Bursaphelenchus xylophilus[J]. Development Growth and Regeneration, 2004, 46(2): 153-161. DOI:10.1111/j.1440-169X.2003.00734.x.
[13] 杨振东. 模式生物秀丽隐杆线虫评价真菌毒素毒性毒理机制的研究[D]. 无锡:江南大学, 2016. YANG Z D. Study of mycotoxins toxic effects and mechanisms using a model organism nematode Caenorhabditis elegans[D]. Wuxi:Jiangnan University,2016.
[14] NOATYNSKA A, GOTTA M. Cell polarity and asymmetric cell division: the C. elegans early embryo[J]. Essays in Biochemistry, 2012, 53: 1-14. DOI:10.1042/bse0530001.
[15] DOLINSKI C, BALDWIN J G, THOMAS W K. Comparative survey of early embryogenesis of Secernentea(Nematoda), with phylogenetic implications[J]. Canadian Journal of Zoology, 2001, 79(1): 82-94. DOI: 10.1139/z00-179.

Last Update: 2019-11-30