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两株我国南北松材线虫虫株形态指标与致病力比较(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年06期
Page:
18-24
Column:
专题报道
publishdate:
2019-11-25

Article Info:/Info

Title:
Comparison of morphological index and pathogenicity of two isolates of Bursaphelenchus xylophilus in southern and northern in China
Article ID:
1000-2006(2019)06-0018-07
Author(s):
SHENG Ruocheng1 LI Min1 CHEN Jun2 GAO Zhijian2 SUN Shouhui3 YE Jianren1CHEN Fengmao1*
(1.Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 2. Fengcheng City Forestry Pest control and Quarantine Bureau of Liaoning Province, Fengcheng 118100, China; 3. College of Forestry, Shenyang Agricultural University, Shenyang 110866, China)
Keywords:
Bursaphelenchus xylophilus[pine wood nematode(PWN)] pathogenicity reproductive capacity temperature adaptation pine wilt disease(PWL)
Classification number :
S763
DOI:
10.3969/j.issn.1000-2006.201907023
Document Code:
-
Abstract:
【Objective】 The pine wood nematode, Bursaphelenchus xylophilus, is an invasive species that damages pine trees, with significant ecological and financial consequences around the world. In China, the species has recently breached its optimal temperature line and has now spread to several districts in Liaoning Province. In this paper, we outline the reasons for the rapid spread of B. xylophilus to northern China by compiling evidence about its temperature adaptability. We compare two isolates, from southern and northern China, to understand differences in pathogenicity and reproductive ability. 【Method】 We compared the morphological index values of the southern B. xylophilus strain AMA3 with the northern B. xylophilus strain FCBX. We tested the virulence and reproductive ability of both strains of B. xylophilus in Pinus thunbergii material to assess its adaptability. We used a culture of Botrytis cinereal, a fungus which contributes to pine disease, to simulate field pathogenicity conditions, under different temperature conditions.【Result】There were no significant differences in the morphological index values of AMA3 and FCBX, all of which were typical of Umbellifera nematodes. Both strains were cultured with B. cinereal in the temperature range of 15-35 ℃, and we found that increases in temperature correlated with increases in reproduction. Reproduction of FCBX strains was generally higher than that of AMA3 strains, although this was reversed at -30 ℃. At low temperatures(15-20 ℃), FCBX produced significantly more replicates than AMA3 did. After vaccination against AMA3 and FCBX, P. thunbergii specimens had different susceptibility to disease, although FCBX maintained a higher relative pathogenicity than AMA3. Thirty-five days after inoculation, P. thunbergii specimens exhibited a mortality of 80% when infected with FCBX, and the disease index was 85; samples infected with AMA3 had a mortality of 60%, and the disease index was 73. Over this period, FCBX reproduced more than AMA3 strains in the P. thunbergii samples, but this difference was not significant. 【Conclusion】 Compared with southern strains(AMA3), northern B. xylophilus strains(FCBX)had slightly larger morphological indicators, higher reproductive capacity in B. cinereal, activity ability, higher reproduction in P. thunbergii, and greater pathogenicity. As B. xylophilus has moved northward, it has been subject to low temperature conditions, and we suggest it has acclimatized to these conditions. It has now broken through its optimal temperature line, and northern strains exhibit strong reproductive capacity, high pathogenicity and adaptability to low temperature conditions.

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Last Update: 2019-11-30