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碳源对不同产地香樟体细胞胚胎发生及植株再生的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2020年01期
Page:
54-62
Column:
研究论文
publishdate:
2020-01-15

Article Info:/Info

Title:
Effects of different carbon sources on somatic embryo of Cinnamomum camphora L.
Article ID:
1000-2006(2020)01-0054-09
Author(s):
JING Ruyue1 WANG Peilan1 HUANG Zhen2HE Linjun1LI Zhihui1*
(1.College of Forestry, Central South Forestry University of Science and Technology, Changsha 410004, China; 2.Sichuan Academy of Forestry Sciences,Chengdu 610081, China)
Keywords:
Cinnamomum camphora L. carbon sources somatic embryo induction rate bud germination rate rooting rate
Classification number :
S792.23
DOI:
10.3969/j.issn.1000-2006.201904002
Document Code:
A
Abstract:
【Objective】 In this study, we investigated the effects of somotic adjustment substances on somatic embryogenesis and regeneration of Cinnamomum camphora L. plants, which will provide a foundation for enhancing the induction rate of somatic embryogenesis and optimizing a somatic embryogenesis system for this species.【Method】The immature zygotic embryos of C. camphora collected in early July in Chenzhou, Changsha, Loudi and Huaihua City were used as materials for examining the effects of different carbon source types, concentrations, and combinations on somatic embryogenesis. The somatic embryo induction rate, germination rate, and rooting rate of plants were determined under different conditions.【Result】The suitable medium for somatic embryo induction was MS medium+1.0 mg/L 6-benzylaminopurine(6+BA)+0.3 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D)+1.0 mg/L streptomycin+15 g/L sucrose+30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable proliferation medium was MS+15 g/L sourse +30 g/L sorbitol+6.5 g/L agar+700 mg/L hydrolyzed casein; a suitable medium for bud germination was murashige and skoog medium+0.1 mg/L thidiazuron(TDZ)+0.5 mg/L naphthylacetic acid(NAA)+0.1 mg/L 3-indolebutyric acid(IBA)+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar; and a suitable medium for rooting was MS+1.0 mg/L IBA+15 g/L sucrose+15 g/L sorbitol+6.5 g/L agar.【Conclusion】 As a consequence of modification, somatic embryo induction was significantly improved by adding 15 g/L sucrose and 30 g/L sorbitol as combined carbon sources, and the new plants thus generated had a larger number of rooting strips, obvious main roots, and better growth characteristics

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Last Update: 2020-01-15