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青钱柳产黄酮类物质真菌的分离与鉴定(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2020年02期
Page:
26-34
Column:
专题报道(执行主编 李维林)
publishdate:
2020-03-31

Article Info:/Info

Title:
Isolation and identification of endophytic fungi from Cyclocarya paliurus (Batal.)Iljinskaja
Article ID:
1000-2006(2020)02-0026-09
Author(s):
KOU Xiaolin1 XIE Nan1 WU Caie12* FAN Gongjian1 FU Xiangxiang2
(1. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China)
Keywords:
Cyclocarya paliurus(Batal.)Iljinskaja flavonoids endophytic fungi ITS analysis scanning electron microscope isolation identification
Classification number :
S718.81
DOI:
10.3969/j.issn.1000-2006.201812001
Document Code:
A
Abstract:
【Objective】This study aimed to identify the endophytic fungi produced flavonoids in Cyclocarya paliurus (Batal.)Iljinskaja and explore a new method to extract flavonoids. 【Method】The endophytic fungi in the branches, leaves, roots, and bark of C. paliurus (Batal.)Iljinskaja were isolated using the tissue separation method. By observing the morphological characteristics of the endophytes, the fermentation products of the endophytic fungi from C. paliurus (Batal.)Iljinskaja were identified using the chromogenic reaction method, thin-layer chromatography(TLC)and high performance liquid chromatography(HPLC). The endophytic fungi produced flavonoids were screened, and the total flavonoid production was determined. Moreover, the strains were identified based on the morphological characteristics and the phylogenetic analysis of the intervening sequences of the fungal rDNA. 【Result】In total, 67 strains of endophytic fungi were obtained; through the identification of morphological characteristics, they were identified to belong to three classes, four orders, six families and ten genera. Four endophytic fungi producing flavonoids were finally identified: PZ06, CY12, CP06 and PP03. The most abundant flavonoids, with a yield of 3.61 mg/L, were produced by strain PZ06. Molecular identification combined with morphological identification indicated that PZ06, CP06 and PP03 belong to the genus Alternaria, whereas CY12 belongs to the genus Eupenicillium. The dominant flavonoid-producing endophytic fungi of C. paliurus (Batal.)Iljinskaja belong to the genus Alternaria. 【Conclusion】 The study of endophytic fungi in C. paliurus (Batal.)Iljinskaja is a key significance for the development and utilization of endophytes. In addition to identifying the endophytic fungi, the present study also provides a novel method for the extraction of active substances from C. paliurus (Batal.)Iljinskaja plants

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Last Update: 2019-03-25