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美洲黑杨I-69树皮中多酚氧化酶同工酶的提纯和特性(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
1991年02期
Page:
16-21
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
PURIFICATION AND PROPERTIES OF POLYPHENOLOXIDASE ISOZYMES FROM BARK OF POPULUS DELTOIDES I 69
Article ID:
1000-2006(1991)02-0016-06
Author(s):
Ji Kungshu Lu Xianhui
Department of Forestry
Keywords:
Populus deltoides 1-69 Bark Polyphenoloxidase Isozyme
Classification number :
-
DOI:
10.3969/j.jssn.1000-2006.1991.02.004
Document Code:
A
Abstract:
Tow polyphenoloxidase isozymes (ppo A and B) in crude extract from populus deltoides 1-69 bark were purified by ammonium sulfate fractionation. Sephadex C- 100 and DEAE-cellulose column chromatography to homogeneity. The data showed that final purification of ppo A and B obtained were 6. 7 and 5. 9 fold. Photographs ofpolyacrylamide dise gels stained with 0. 03 m catechol and 2% coomassie blue R-250 after electronphoresis of ppo A and B preparation showed single band, respectively. Molecular weight of ppo A and B determined by sodium dodecy 1.sulfate polyacryamide get electronphoresis was 38900 and 33000. From the amino acid composition, it was found that ppo A molecular contanied most large amounts of lysine (residue 86), least amounts of arginine (residue 3) and did not contain tyrosine, and proline, ppo B molecular contained largest amounts of serine (residue 37), least amounts of arginine (residue 3) and did not contain tyrosine. The ppo A and B gave pH optima of 6. 4 and 7. 0 and temperature optima of 50 C and 45 C.Thermostability of ppo A was relatively stable as compured with ppo B. Of a number of phenolic compound tested, Km value of ppo A and B for catechol was least (0. 39 and 0. 34 mM), they had largest affinity for catechol. Therefore, ppo A and B was catecholase. In a number of compound tested, 1mM L-systein, sodium thiosulfate, thiourea, sodium dithyldithiocarbamete, sodium chloride and sucrose produced an inhibiting effect on activity of ppo A and ppo B, 0.1mM sodium chloride and sucrose caused promotive effect of activity of ppo B.

References

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Last Update: 1900-01-01