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墨天牛属三个近缘种的RAPD分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
1998年04期
Page:
35-38
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
RAPD ASSESSMENT OF THREE SIBLING SPECIES OFMONOCHAMUS GUER (COLEOPTERA: CERAMBYCIDAE)
Article ID:
1000-2006(1998)04-0035-04
Author(s):
An Yulin Diao Caihua Zhu Hongbing Jiao Guoyao
Nanjing Animal and Plant Quarantine Service Nanjing 210009
Keywords:
Monochamus Guer Sibling Species Larvae RAPD Identification
Classification number :
-
DOI:
10.3969/j.jssn.1000-2006.1998.04.008
Document Code:
A
Abstract:
Three sibling species, Monochamus alternatus Hope, M. urussovi Fischer and M. sutor Linnaeus were analysed by using randomly amplified polymorphic DNA (RAPD) method. The result indicated that three primers were evident to separate those three sibling species. Whereas those in different stages of same species were almost of no difference. DNA pured from soaked and live individuals of cerambycid larvae for RAPD reactions using several primers resulted in identical RAPD fingerprints. Consequently, it is an available method newly provided by the authors for identification of sibling cerambycid species, especially for cerambycid larvae.

References

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Last Update: 1900-01-01