我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

从针叶树营养体组织中提取高纯度DNA的方法(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
1998年04期
Page:
87-90
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
DNA EXTRACTING METHODS FROM VEGETATIVE TISSUE OF CONIFER
Article ID:
1000-2006(1998)04-0087-04
Author(s):
Ying Tongming Han Zhengmin Huang Minren
College of Forest Resources and Environment Nanjing Forestry University Nanjing 210037
Keywords:
Vegetative tissue of conifer DNA isolation Method of SDS Method of CTAB
Classification number :
-
DOI:
10.3969/j.jssn.1000-2006.1998.04.019
Document Code:
A
Abstract:
The quality of the isolated DNA is one of the most important facts that influence the result of DNA analysis. Some experiments, such as genomic mapping and population genetics require preparation of large numbers of DNA samples that are correctly identified with respect to the individuals that were the source of the DNA samples. The identity of samples must be maintained, but the samples must be processed quickly and efficiently. Here we describe a simple method to isolate high-quality and identified DNA from vegetative tissue of conifer. The crucial step is to select the suitable material. Different from the common used tissues, whose yielded DNAs are often degraded due to its complicated secondary substance and additive, the tender unwoody terminals are adopted for DNA isoaltion. Here also describes two DNA isolation protocols. According to these protocols, DNA yields and purities were similar for all samples. Highly purified DNA from different conifer species can be obtained following these procedures.

References

-

Last Update: 1900-01-01