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中国马褂木精细胞分离技术(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
1999年03期
Page:
12-15
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Mass Isolation of Viable Sperm Cells from the Pollen of Liriodendron chinense
Article ID:
1000-2006(1999)03-0012-04
Author(s):
Author Zhou JianFan Ruwen
College of Forest Resources and Environment Nanjing Forestry UniversityNanjing210037
Keywords:
Liriodendron chinense Sperm cell Mass isolation
Classification number :
-
DOI:
10.3969/j.jssn.1000-2006.1999.03.005
Document Code:
A
Abstract:
Since 80’s,the isolation and biological studies of sperm cells in angiosperms have been an important research area in plant fertilization engineering. Based on the isolation of vialbe sperm of Liriodendron chinense, the mass isolation of viable sperm cells form the its pollen was reported. With sugar density gradient centrifugation method, the germinated pollen was firstly purified. And the vialbe sperm were released by a means of the grinding method. The viable sperm was purified with sugar density gradient centrifugation. The testing result shows that the isolated sperm cells might survive for 4h at room temperature.

References

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Last Update: 1900-01-01