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荧光假单胞菌产木聚糖酶的纯化和性质(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2000年02期
Page:
60-62
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Purification and Properties of Xylanase from Pseudomonas flu.
Article ID:
1000-2006(2000)02-0060-03
Author(s):
Su Yuping\ Wang Shuhan\ Ou Kai
College of Chemical Engineering Nanjing Forestry University\ Nanjing 210037
Keywords:
Pseudomonas flu. ; Xylanase; Purification; Michaelis constant
Classification number :
Q556
DOI:
10.3969/j.jssn.1000-2006.2000.02.018
Document Code:
A
Abstract:
Using the systematical process for isolation and purification of xylanase from Pseudomonas flu. with the tehnique (NH) 2SO 4 fractionation and Sephede x G100 gel column, we obtained the xylanase showing homogeneity in the gel electrophorosis.The melecular weight was 33?000 by SDS polyacrylamide.The optimum pH,temprature were 6.0 and 60?℃,respectively.The studies of enzyme kinetics showed K m=4.76?mg/mL.

References

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Last Update: 1900-01-01