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防止黑松外植体及其愈伤组织褐变的方法(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2001年05期
Page:
75-77
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Methods to Prevent Explant and its Calli from B rowning in Tissue Culture in Pinus thunhergii Parl.
Article ID:
1000-2006(2001)05-0075-03
Author(s):
GAO RongZHAO Bo-guang
College of Forest Resources and Environment Nanjing Forestry U niversity, Nanjing 210037,China
Keywords:
Pinus thunhergii Parl.ExplantCalliBrowning
Classification number :
Q945
DOI:
10.3969/j.jssn.1000-2006.2001.05.019
Document Code:
A
Abstract:
The methods to preventing the explant and its calli from browning in the processes of vitro propagation of Pinus thunhergii were stu died.The result showed that mature embryo of P.thunhergii is a better explan t than new shoot for its lower browning death rate.Culturing at dark is a necess ary condition for avoiding browning.Adding Vc and active carbon cannot overcome the browning of the calli.Selecting suitable medium (1/2MS) and using proper ho rmones at certain concentrations could prevent browning and increase growth effe ctively.

References

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Last Update: 1900-01-01