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银杏、板栗不同组织DNA的提取(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2001年06期
Page:
80-82
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
DNA Extraction from Different Tissues of Ginkgo and Chinese Chestnut
Article ID:
1000-2006(2001)06-0080-03
Author(s):
SHEN Yong-bao.SHI Ji-sen
Southern Forest Tree Seed Center of National Forestry Bureau, Nanjing 210037,China
Keywords:
DNA extractionGinkgoChinese chestnut
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2001.06.021
Document Code:
A
Abstract:
This paper shows that the genomic DNA from different tissues of Ginkgo and Chinese chest- nut was extracted by CTAB method. The results indicate that except for mature leaf, other tissues, uch as dormant bud, young leaf, phloem and half-lignous phloem, can be used to extract DNA easily and successfully. In addition,the value of A260/A280 for each DNA samples above mentioned is over 1. 8,and the quantity is over 22~50 g per 100 mg. Also,clear and bright products of RAPD reveal that the DNA may meet needs of DNA molecular markers.

References

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Last Update: 1900-01-01