我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

厚朴组织培养与高产细胞系建立的研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2002年04期
Page:
23-26
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Studies on Tissue Culture and the Establishment of a High-Yield Cell Line of Magnolia officinalis
Article ID:
1000-2006(2002)04-0023-04
Author(s):
TONG Zai kang 12 ZHU Yu qiu 2 WANG Zhang rong 1
1.Nanjing Forestry University,Nanjing 210037,China;2.Zhejiang Forestry College,Lin’an 311300,China
Keywords:
Magnolia officinalis Tissue cultureCallusCell cultureMagnololHonokiol
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2002.04.006
Document Code:
A
Abstract:
In this paper,techniques for induction of callus and cell culture in Magnolia officinalis have been studied,with an aim to establish a cell line in which cells proliferated fast and phenol content was high.Based on tissue culture and the establishment of a high yield cell line of 10 genotypes from a provenance test forest of Magnolia officinalis ,it shows that such factors as basic media,types and proportions of hormones,and explants had a great effect on the induction rate,growth and browning of the callus.With a medium of B 5 +4 mg/L 2,4 D+1 mg/L NAA,the induction rate reached 100%.With the medium of B 5 +1.2-2.0 mg/L BA+1.0 mg/L NAA,the callus had a highest proliferation rate and a lowest percentage of browning.Cells of the two genotypes, viz.140 and 54 grew fastest and were 2.12 times as many as those of other genotypes on the average.The content of phenols differed between calluses cultured from different genotypes and media as well as for a different period of time.The best one was the cell line of the genotype 140 cultured on the medium of B 5 +2.0 mg/L BA+ 1.0 mg/L NAA for 60 days.

References

-

Last Update: 1900-01-01