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非洲菊的离体培养及其快速繁殖(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2003年02期
Page:
33-36
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Tissue Culture and Rapid Propagation of Gerbera jamesonii
Article ID:
1000-2006(2003)02-0033-04
Author(s):
XI MengliSHI Jisenng NanjingForestry UniversityNanjing 210037China)
Keywords:
Gerbera jamesonii BolusTissue culture Rapid propagation
Classification number :
Q945.5
DOI:
10.3969/j.jssn.1000-2006.2003.02.008
Document Code:
A
Abstract:
The results of tissue culture and rapid propagation on 4 Gerbera cultivars were reported. Buds were induced from 0.5~1.0 cm long young leaves cultured on MS medium supplemented with 2.0 mg/L 6BA and 0.1 mg/L NAA for 12~21 days,with an induction rate higher than 60%.It was a best way for adventitious bud regeneration.Three kinds of media:viz.MS+0.3 mg/L 6BA +0.1 mg/L NAA,MS+1.0 mg/L 6BA+0.1 mg/L NAA and MS+0.3 mg/L 6BA+0.1 mg/L PP333+0.1 mg/L NAA were used alternatively for propagation of bud subculture,and the average propagation coefficient was 5.1.The medium of MS+0.1 mg/L PP333+0.1 mg/L NAA were used for rooting,and the rooting rate was more than 95%.Rooted plantlets could survive after 15~20 days when they were transferred to peat.They could be planted in field after 25 days and could flower after 3~4 months.An efficient and rapid production system has been successfully developed to propagate Gerberas.

References

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Last Update: 1900-01-01