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塑料半薄切片法观察豆科植物根表面传递细胞(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2003年02期
Page:
65-68
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Observing the Transfer Cells in the Epidermal Cells of Rootsof Leguminous Plants with Semi-thin Sections
Article ID:
1000-2006(2003)02-0065-04
Author(s):
WU JunzhangHAN SufenCollege of Forest Resources and Environment Nanjing Forestry UniversityNanjing 210037China)
Keywords:
Transfer cellsGMASemi-thin sectionOil subjetive
Classification number :
S188
DOI:
10.3969/j.jssn.1000-2006.2003.02.016
Document Code:
A
Abstract:
For observing the transfer cells in epiderm of the roots by optical microscope,the method is taken as follow:after the leguminous plants Albizia julibrissin,Astragalus sinicus,Medicago sativa induced by Rhizobia,their root segments where root hair grows densely and swells a little are selected to be fixed in FAA.Through a series of treatment such as being embedded in GMA,semithin sections(3 μm),staining with PAS reaction,the root cross sections were observed by optical microscope under the oil objective.The result shows that on the tangential exowall of the epidermal cells or exodermis of the roots,the wall ingrowth is easy to be found.It is the typical epidermal transfer cells of root.This method is not only simple with a low cost but also easy to determine the position of the transfer cells in the root.

References

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Last Update: 1900-01-01