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杉木单染色体扩增产物的RAPD分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2003年03期
Page:
55-58
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
RAPD Studies on the Amplified Product of Single Microdissected Cunninghamia lanceolata Chromosome
Article ID:
1000-2006(2003)03-0055-04
Author(s):
LI Xiangyang1ZHOU Jian1*YUAN Hansheng2ZHANG Zailiang2
1.Nanjing Forestry University,Nanjing 210037,China;2.Forestry Bureau of Yongzhou Hunan Province,Yongzhou 425000,China
Keywords:
Cunninghamia lanceolataMicrodissectionDOP-PCRRAPD
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2003.03.013
Document Code:
A
Abstract:
Four single chromosome of Cunninghamia lanceolata was dissected under the micromanipulator.After degenerate oligonucleotideprimedPCR amplification(DOPPCR),DNA product were tested with RAPD.The results indicated: the polymorphism among different single microdissected Cunninghamia lanceolata chromosome was evident.This research provides a new method to identify microdissected Cunninghamia lanceolata chromosome and locate gene to chromosome of Cunninghamia lanceolata chromsome.

References

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Last Update: 1900-01-01