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一种高效的树木总DNA提取方法(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2004年01期
Page:
13-16
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
A Rapid and Simple Method of Total DNA Extraction from Tree
Article ID:
1000-2006(2004)01-0013-04
Author(s):
ZHANG Bo~1ZHANG Lu~(12)ZHUGE Qiang~1WANG Ming-xiu~1HUANG Min-ren~(1*)
1.Nanjing Forestry University,Nanjing 210037,China;2.Jiangxi Agricultural University,Nanchang 330045,China
Keywords:
DNASilica particles(SiO_2)Guanidinium thiocyanate(GuSCN)
Classification number :
Q343.15
DOI:
10.3969/j.jssn.1000-2006.2004.01.003
Document Code:
A
Abstract:
High quality DNA samples are one of key factors in study of forest molecular biology.Based on the presence of high concentrations of the chaotropic agent guanidinium thiocyanate(GuSCN),the nucleic acid could bind to silica particles(SiO_2) and subsequently was eluted in a aqueous low-salt buffer.A new method of total DNA extraction from leaves of tree was established.The method is rapid and simple.High quality DNA could be isolated from leaves of (5 tree) species proved by the concentrations,purities,PCR amplification and restriction digests.This method could be used widely in study of forest molecular biology.

References

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Last Update: 1900-01-01