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杨树黑斑病感抗无性系cDNA文库的构建(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2004年03期
Page:
83-85
Column:
研究论文
publishdate:
1900-01-01

Article Info:/Info

Title:
Construction of cDNA Libraries with Leaves of Clones Susceptible and Resistant to Black Spot Disease in Poplar
Article ID:
1000-2006(2004)03-0083-03
Author(s):
ZENG Yan-ru~(12)HUANG Min-ren~(1*)WANG Ming-xiu~1
1.Nanjing Forestry University,Nanjing 210037,China;2.Zhejiang Forestry College,Lin’an 311300,China
Keywords:
Populus deltoids cv.’Lus’P.euramericana CL.Black spot disease in poplarcDNA library
Classification number :
Q78
DOI:
10.3969/j.jssn.1000-2006.2004.03.021
Document Code:
A
Abstract:
In order to isolate target genes associated with disease resistance,two cDNA libraries viz.L45-72 and L69-72 were constructed on the basis of anti-disease cDNA fragments obtained by fluorescent DDRT-PCR with ready-to-use kits and pathogen-inoculated poplar leaves of I-69 and I-45,two clones either extremely resistant or susceptible to black spot disease in poplar.The library of I-45(L45-72) had a titer of 3.44×10~9 pfu/mL and the library of I-69(L69-72) had a titer of 3.94×10~9 pfu/mL,with inserts larger than 500 bp accounting for (96.67%) and 73.33%,respectively.The libraries constructed have laid a foundation for studies on the mechanism of disease resistance and disease susceptibility.

References

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Last Update: 1900-01-01