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蝴蝶兰叶片诱导植株再生的研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2005年03期
Page:
28-32
Column:
研究论文
publishdate:
2005-03-20

Article Info:/Info

Title:
Plantlets Regeneration from Leaf Explants of Phalanopsis
Article ID:
1000-2006(2005)03-0028-05
Author(s):
LI Jie12 HUANG Min-ren1 WANG Ming-xiu1 CAI Ru2
1. The Key Laboratory of Forestry Genetics and Gene Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Orchids Engineering Research Centre of Jiangsu Province, Taizhou 225321, China
Keywords:
Phalanopsis Leaf explants Plantlets regeneration
Classification number :
S682.31
DOI:
10.3969/j.jssn.1000-2006.2005.03.007
Document Code:
A
Abstract:
Plantlets regeneration from leaf explants of Phalanopsis was studied. The results showed that:(1) Decontamiated-materials could be obtained by inducing from floral stalk, and proliferation of plantlet was achieved on Murashige and Skoog(MS) medium supplemented with 6-BA(7. 5 mg/L)+NAA(1.0 mg/L); (2)6-BA and NAA can stimulate PLBs(protocorm-like bodies) formation and the effective ruslts could be obtained on MS 6-BA(10.0 mg/L)+NAA(1.0 mg/L); (3)The optimal mediums were 1/2MS and Hyponex mediums for proliferation and transformation of PLBs; (4)The relations between the concentration of sucrose and plantlets regenerations from PLBs could be formulated by y=-0. 019 92 x2+0. 077 51 x+0. 170 00, and plantlets developed well on 1/2 MS containing 20 g/L without any growth regulators.

References

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Last Update: 2013-05-20