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赤枝叶片的离体培养与植株再生(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2005年03期
Page:
45-48
Column:
研究论文
publishdate:
2005-03-20

Article Info:/Info

Title:
In Vitro Plant Regeneration from the Seedling Leaves of Eucalyptus camaldulensis
Article ID:
1000-2006(2005)03-0045-04
Author(s):
GUAN Ya-li HUANG Min-ren WANG Ming-xiu
The Key Laboratory of Forestry Genetics and Gene Engineering Nanjing Forestry University, Nanjing 210037, China
Keywords:
Eucalyptus carnaldulensis Leaf Adventitious shoot inducing Plant regeneration
Classification number :
S723
DOI:
10.3969/j.jssn.1000-2006.2005.03.011
Document Code:
A
Abstract:
In vitro seedling leaves were used as explants to study the shoot inducing and plant regeneration of E. camaldulensis. The inducing rate of the adventitious shoot was 35. 4% when the explants were cultured on medium of MS+0. 8 mg/L BA+0. 3 mg/L KT+0.05 mg/L NAA,but the inducing rate could be stimulated up to 42.9% when medium of MS+0. 8 mg/L BA+0.05 mg/L TDZ+0. 1 mg/L NAA was used. A ten-day darkness culture could improve the adventitious shoot inducing and the explants from more tender leaves were easier to induce the shoots. The shoots with 2~3 cm length were cultured on the rooting medium and the rooting rate of 100% could.be obtained 30 days later. The transplanting rates of rooted shoots were over 80%.

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Last Update: 2013-05-20