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NP1和GAFP双价抗病基因植物表达载体的构建(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2005年06期
Page:
7-10
Column:
研究论文
publishdate:
2005-10-20

Article Info:/Info

Title:
The Construction of Two’ Disease-resistance Gene NP1 and GAFP Vector
Article ID:
1000-2006(2005)06-0007-04
Author(s):
CHEN Ying1 DAI Yong-mei12 WANG Yi-qin3 SUN Yong-ru3 HUANG Min-ren1 WANG Ming-xiu1
1. The Key Laboratory of Forest Tree Genetics and Engineering State Forestry Administration Nanjing Forestry University, Nanjing 210037, China; 2. Shanghai Flower Breeding Centre, Shanghai 200072, China; 3. Institute of Genetics and Development Biology, CAS, Beijing 100101, China
Keywords:
Defensin NP1 Gastrodia antifungal protein (GAFP) Two antidisease genes Vector construction
Classification number :
S722.3; Q813
DOI:
10.3969/j.jssn.1000-2006.2005.06.002
Document Code:
A
Abstract:
Defensin is resistant to many kinds of virus, bacterium and fungusl. Gastrodia Antifungal Protein (GAFP) has the resistant ability to more than twenty kinds of fungi. The antidisease mechanisms and spectrum of the two genes are different. Using DNA reconstruction method a vector (pBin35SGAFPNP1) that contains two antidisease genes: NP1 and GAFP were constructed. Then two disease-resistance genes could be transferred into the same plant just through one time of transformation that can improve the disease-resistant ability of the transgenic plant to more kinds of pathogens. So the proceeding of plant antidisese breeding could be accelerated.

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Last Update: 2013-05-20