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杉木合子胚愈伤组织的诱导及植株再生

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年02期
Page:
6-10
Column:
研究论文
publishdate:
2006-02-20

Article Info:/Info

Title:
Callus Induction and Plantlets Regeneration from Zygotic Embryos of Cunninghamia lanceolata Hook.
Article ID:
1000-2006(2006)02-0006-05
Author(s):
XI Meng-li SHI Jisen*
The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University, Nanjing 210037, China
Keywords:
Cunninghamia lanceolata Hook. Callus Plantlets regeneration
Classification number :
S722.3+7
DOI:
10.3969/j.jssn.1000-2006.2006.02.002
Document Code:
A
Abstract:
Using mature zygotic embryos and immature zygotic embryos as initial explants, the effect of the following factors on induction callus and differentiation adventitious buds.. basal medium, plant hormone and development stages of zygotic embryo were studied. Results indicated that the optimal medium for mature zygotic embryos induction callus was 1/2MS+2mg/L 2,4-D+1.0mg/L 6-BA+1. 0mg/L KT+30 g/L sucrose. This medium can induce soft, transparent callus from immature zygotic embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2%~6%. Immature zygotic embryos of tissue and organ differentiation or embryo mature stages induction callus were similar in state with those of mature zygotic embryo. The callus induction frequency was significantly improved with the maturity of immature zygotic embryos. The optimal medium for differentiation adventitious buds was DCR+1.5mg/L 6-BA+1. 5mg/L KT+20 g/L sucrose. Shoots with 1. 5 cm high were transfered to rooting medium and rooting frequency reached 100%.

References

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Last Update: 2013-05-20