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黑松丛生芽的诱导及植株再生(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年03期
Page:
27-31
Column:
研究论文
publishdate:
2006-03-20

Article Info:/Info

Title:
Axillary Buds Induction and Plantlet Regeneration of Pinus thunbergii
Article ID:
1000-2006(2006)03-0027-05
Author(s):
ZHU Li-hua Zheng Dan WU Xiao-qin*
College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China
Keywords:
Pinus thunbergii Seedling Axillary bud induction Plantlet regeneration
Classification number :
S718; Q943.1
DOI:
10.3969/j.jssn.1000-2006.2006.03.005
Document Code:
A
Abstract:
A plantlet regeneration protocol was developed for Pinus thunbergii. The procedure involves 3 steps, axillary buds induction, buds growth and root induction. Axillary buds induction was found to be influenced by the concentration of 6-benzyladenine (6-BA) and alpha-naphthylacetic acid (NAA) in the induction medium as well as the age of the seedling from which the explant was taken. Of the various 6-BA and NAA combination tested, modified Gresshoff and Doy’s medium (GD) with 4 mg/L 6-13A and 0.05 mg/L NAA proved to be the most effective, and the right stage of seedling was 22 ~ 28 d. During the next 5 weeks of culture on the same 6-BA-free but with 0.1 mg/L NAA medium multiple shoots elongated from the buds. The medium containing GA3 did not support buds growth. Elongated shoots were transferred to half-strength WPM medium supplemented with 0.2 mg/L NAA for root initiation and 14 days later transferred to half-strength WPM medium without any hormone for root development. After 1 month, 23.3 ~ of the shoots had formed adventitious roots.

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Last Update: 2013-05-20