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福建山樱花不定芽诱导和植株再生规模化繁殖试验

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年03期
Page:
105-108
Column:
研究论文
publishdate:
2006-03-20

Article Info:/Info

Title:
Adventitious Bud Inducing and Plant Regeneration of Cerasus campanulata Maxim. in Large Scale
Article ID:
1000-2006(2006)03-0105-04
Author(s):
Lv Yue-liang12 CHEN Zhang2 SHI Ji sen1* HUANG Yu-xiang3 LIU Jin-yan3 XIE Jian-li3
1. The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu ProvinceNaniing Forestry University, Nanjing 210037, China; 2. Forestry Administration of Fujian Province, Fuzhou 350003, China; 3. Crop Institute of Fujian Academy of Agriculture, Fuzhou 350013, China
Keywords:
Cerasus campanulata Tissue culture Adventitious bud Plant regeneration Masspropagation
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2006.03.024
Document Code:
A
Abstract:
The explants in 4 em length collected from spring shoots of 3-year-old selected tree of Cerasus carnpanulata were inoculated on MS culture medium with 6-BA and IBA for adventitious buds mutiplication. The results showed that explants were prevented effectively from being brown and dying when the medium of 1/4MS were used. Explants grew fast and no vitrification when the medium plused with 1.0 mg/L 6-BA and 0.01 mg/L IBA. The medium of MS with 1.0 mg/L 6-BA and 0.3 mg/L GA3 was the best for multiplication and multiplication rate could reach to 600% during one culture cycle. Plantlets were to be transferred on the 1/2MS medium with 1.0 mg/L NAA and 0.2 mg/L IBA for rooting, when the explants were reached in about 4 cm. The root system could be developed well during 25 days and to be come a new plant. The rooting rate could reach to 92.1% after 35 days. The survival rate was above 94% when the plantlets were to be moved to nursery. The medium in 340//00 pearlite and 67%peat soil was good for rooting. This micropropagation technology could be applied in mass propagation.

References

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Last Update: 2013-05-20