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植物冰冻切片技术的改进(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年03期
Page:
128-130
Column:
研究简报
publishdate:
2006-03-20

Article Info:/Info

Title:
Improvement of Plant Cryo-sectioning Technique
Article ID:
1000-2006(2006)03-0128-03
Author(s):
LIU Jian-feng1 CHENG Yun-qing1 YAN Xiu-feng2 ZHOU Ge1 WANG Shu-fan3
1. School of Life Sciences Jilin Normal University, Siping 136000, China; 2. College of Life Sciences Northeast Forestry University, Harbin 150040, China; 3. Landscape Office of Siping Jilin Province, Siping 136000, China
Keywords:
Cryo-sectioning Technique improvement Schisandra chinensis (Turez.) Ball.
Classification number :
S718.3; Q94-3
DOI:
10.3969/j.jssn.1000-2006.2006.03.030
Document Code:
A
Abstract:
It was a time consuming process if technique of paraffin section was used to observe plant specimen because fussy processes such as dehydrating, infiltrating and embedding were needed before being observed. Cryo-sectioning technique was used to observe leafstalk, leaf, stem and root structure of Schisandra chinensis (Turez.)Ball.. The operating process was simple and efficient. Clear image could be acquired within one day. The crucial step were to select a proper concentration of glycerol, a suitable temperature of freezing and an appropriate way of section-flatting.

References

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Last Update: 2013-05-20