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杉木遗传转化受体系统的建立(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年06期
Page:
7-11
Column:
研究论文
publishdate:
2006-06-20

Article Info:/Info

Title:
Establishment of Receptor System for Genetic Transformation of Cunninghamia lanceolata Hook.
Article ID:
1000-2006(2006)06-0007-05
Author(s):
XI Meng-li SHI Ji-sen*
The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University, Nanjing 210037, China
Keywords:
Cunninghamia lanceolata Hook. Organogenesis Receptor system
Classification number :
S722.3+7
DOI:
10.3969/j.jssn.1000-2006.2006.06.002
Document Code:
A
Abstract:
Using plantlet shoot sections near the shoot tip as initial explants, we observed the effects of several factors on induction buds, such as pre-culture time, 6-BA, TDZ, Cef, Km. From shoot sections we established a receptor system for genetic transformation. The results indicated that for shoot sections induction buds the optimal medium was DCR+6-BA (1.0mg/L)+TDZ (0.001mg/L)+NAA (0.1mg/L). Pre-cultured for two days, the bud induction frequency, average number of bud and bud forming capacity all reached the maximum, which was 100%, 6.35 and 6.35, respectively. Histological observations indicated that buds originate from the surface of the shoot sections, where Agrobaterium tumefaciens can reach. It was shown that sticking A. tumefaciens cells and developing buds cells share the same position.

References

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Last Update: 2013-05-20