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北美悬铃木的组织培养(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年06期
Page:
61-65
Column:
研究论文
publishdate:
2006-06-20

Article Info:/Info

Title:
Tissue Culture and Mass Propagation of Platanus occidentalis Linn
Article ID:
1000-2006(2006)06-0061-05
Author(s):
ZOU Yong-mei12 SHI Ji-sen1*
1. The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University, Nanjing 210037, China; 2. Department of Biology Jiangsu Institute of Education, Nanjing 210013, China
Keywords:
Platanus occidentalis Linn Tissue culture Explant
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2006.06.014
Document Code:
A
Abstract:
Top cut pruning was made on an American sycamore crown, and segments from new sprout were collected as tissue culture initial explants. The results indicated that as for shoot segments induction buds the optimal medium was MS+NAA (0.1mg/L)+6-BA (1.0mg/L)+sucrose (30g/L), and DCR+NAA (0.1mg/L)+6-BA (1.0mg/L)+KT (0.3mg/L)+sucrose (30g/L) was the optimal medium for induction buds proliferation, on which induction buds grew well and coefficient of multiplication reached 6.4 times. We developed two ways to get adventitious buds to root. One was that adventitious buds were transferred on the DCR medium with 150mg/L R. The other, on the DCR medium with 60mg/L R about for 15d and then, transferred on a garniture-free DCR. By the two ways, both of the rooting percentages were up to 100%.

References

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Last Update: 2013-05-20