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高山红景天花粉的超低温保存(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2006年06期
Page:
138-140
Column:
研究简报
publishdate:
2006-06-20

Article Info:/Info

Title:
The Cryopreservation of Rhodiola sachalinensis A. Bor Pollen
Article ID:
1000-2006(2006)06-0138-03
Author(s):
LIU Jian-feng12 YAN Xiu-feng1* CHENG Yun-qing2 CHEN Yin-hua3
1. College of Life Sciences Northeast Forestry University, Harbin 150040, China; 2. School of Life Sciences Jilin Normal University, Siping 136000, China; 3. College of Life Sciences and Agriculture Hainan University, Haikou 570228, China
Keywords:
Rhodiola sachalinensis A. Bor Pollen Cryopreservation
Classification number :
Q944.42
DOI:
10.3969/j.jssn.1000-2006.2006.06.033
Document Code:
A
Abstract:
Researches had been carried out on cryopreservation of pollen from Changbai Mountain and Daxinanling Mountain. The results indicated that desiccation duration affected the pollen germination after cryopreservation. The germination viability of pollen that was prefrozen for 12h was higher than that of 8h, 24h. Treatment at varied temperate of 0℃ (8h)→-10℃ (8h)→-20℃ (8h) could greatly increase the viability of pollen stored in liquid nitrogen before being thrown into liquid nitrogen. The defreezing method of -20℃ (8h)→4℃ (8h)→25℃ (8h) was better than other two treatments.

References

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Last Update: 2013-05-20