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与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年01期
Page:
15-18
Column:
研究论文
publishdate:
2007-01-20

Article Info:/Info

Title:
Development of SCAR Markers Linked to Resistance Locus of Black Spot Disease in Populus deltoides
Article ID:
1000-2006(2007)01-0015-04
Author(s):
ZHANG Yan-mei PAN Hui-xin ZHANG Bo WANG Ming-xiu HUANG Min-ren*
The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University, Nanjing 210037, China
Keywords:
Poplar black spot disease Random Amplified Polymorphic DNA (RAPD) Sequence-characterized Amplified Regions (SCAR)
Classification number :
S722;Q813
DOI:
10.3969/j.jssn.1000-2006.2007.01.004
Document Code:
A
Abstract:
RAPD markers OPAI17-1550 and OPAI13-900, linked to anti-black-spot disease gene locus, were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13-292 were validated in the resistant、 susceptible pools and F1 population. The SAI17-1570 had the same amplification with templates of the positive OPAI17-1550 F1, population and resistant pool, did not have amplification with templates of the negative OPAI17-1550 F1 population and susceptible pool. The SAI13-292 had one band with templates of the negative OPAI13-900 F1 population and susceptible pool, but two bands with templates of the positive OPAI13-900 F1 population and resistant pool. The two SCAR markers could be of great importance for clone selection on seedling stage.

References

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Last Update: 2013-05-20