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杉木优良无性系组培快繁技术体系的建立(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年03期
Page:
47-51
Column:
研究论文
publishdate:
2007-03-20

Article Info:/Info

Title:
Establishment of Technique system of Tissue Culture on Chinese Fir Superior Clones
Article ID:
1000-2006(2007)03-0047-05
Author(s):
OUYANG Lei2 ZHENG Ren-hua2 WENG Yu-zhen2 CHEN Xiao-chou2 LI Lin-yuan3 KANG Yue-hui1
1. Fujian Academy of Forestry, Fuzhou 350012, China; 2. Fujian Yangkou Forest Farm, Shunchang 353211, China; 3. Fujian Jiangle Forest Farm, Jiangle 3533000China
Keywords:
Chinese fir Superior clone Tissue culture and rapid propagation
Classification number :
S722.3
DOI:
10.3969/j.jssn.1000-2006.2007.03.010
Document Code:
A
Abstract:
Tissue culture technique of 36 plus-tree selected from Chinese fir progeny tests was reported in this paper. Among the 36 plus-tree 5 superior clones were systematically studied and their technique system of tissue culture was established completely. The techniques were as follows: Initial culture media was 1/2MS+6-BA(0.3~0.8mg/L)+Sucrose (3%), clump shoots induction culture media was 1/2MS~6-BA(0.5~0.7mg/L)+IBA(0.1~0.3rag/L)+Sucrose (3%), rooting culture was 1/2 improvement MS+IBA (0.5~1.0rag/L) or 1/2 improvemen, MS+ABT1#(1.0~1.5mg/L)+Sucrose (1.50%), and transplantation media was yellow-center soil 90%+fine sand 10%. Their rooting rate was up to 90%, and transplantation survival rate was up to 96%.

References

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Last Update: 2013-05-20