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磁处理对滨梅组培苗增殖及再生的影响

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年03期
Page:
99-102
Column:
研究论文
publishdate:
2007-03-20

Article Info:/Info

Title:
Effects of Magnetic Treatment of Beach Plum in vitro Subculture Explants on the Regeneration and Growth
Article ID:
1000-2006(2007)03-0099-04
Author(s):
YAN Dao liang1 QIN Pei1* WU Wei-guo2 WANG Guang1 ZAI Xue-ming1 SUN Dong-lin1
1. Halophyle Research Lab of Nanjing University, Nanjing 210093, China; 2. Department of Physics of Nanjing University, Nanjing 210093, China
Keywords:
Magnetic treatment Beach plum In vitro subculture explants Regeneration and growth
Classification number :
S722.3+7
DOI:
10.3969/j.jssn.1000-2006.2007.03.021
Document Code:
A
Abstract:
The magnetic field is applied as a strength gradient (48~115 kA/m). It is shown that the increase in the field strength stimulates the regeneration and growth in the explants. Effects of a ten-minute treatment of explants with a strength of 97 kA/rn on the regeneration and growth are significant, and the number of the sprouts induced is 2.3 times that the number of control group. The fresh weight, dry weight and height of the sprouts, from the explants were treated with a strength of 97 kA/m, in culture medium MS+ZT(2mg/L)+IBA (0.2rng/L)+Vc(30 rag/L) are more significant than those in MS+ZT(2mg/L)+NAA (0.2mg/L)4-Vc(30mg/L) and control group. It is greatly significant at 0.01level that the root length of plantlets from explants treated with a strength of 97 kA/m and put into optimized rooting medium 1/2MS+NAA(0.1mg/L)q-IBA(0.1mg/L)+bovin.e serum albumin 30mg/I., compared with the control group. The average root length is (5.39~0.68)cm. It is possible that the optimization of culture medium and treatment of explants with a certain magnetic field strength can increase the number of regeneration sprouts and can promote growth.

References

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Last Update: 2013-05-20