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一种适合杨树叶片的蛋白质提取方法

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年03期
Page:
119-121
Column:
研究论文
publishdate:
2007-03-20

Article Info:/Info

Title:
A Method Suitable for Protein Extraction of Poplar Leaves
Article ID:
1000-2006(2007)03-0119-03
Author(s):
YUAN Kun WANG Ming xiu HUANG Min-ren*
The Key 1. ahoratory of Foresl Genetics and Gene Engineering of the State Forestry Administration and Jiangsu ProvinceNanjing Forestry University Nanjing 210037. China
Keywords:
Poplar leaves Protein extraction Two-dimensional gel electrophoresis
Classification number :
8722; Q78
DOI:
10.3969/j.jssn.1000-2006.2007.03.027
Document Code:
A
Abstract:
High-quality protein sample preparation is the premise of two-dimensional gel electrophoresis (2-DE). Poplar leaves contain abundant Interfering compounds such as polyphenols, pigments, polysaccharides and so on, which make it difficult to extract proteins. In order to find a method suitable for protein extraction of poplar leaves, the poplar of NI.895 was used as the experimental material. The method of trichloroacetic acid (TCA)-acetone was adopted to extract proteins. Coomassie brilliant blue (CBB) and silver staining were used respectively to detecl the extracted proteins. Good protein patterns were obtained, This paper provides a basis for poplar proteomics research in our lab.

References

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Last Update: 2013-05-20