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3种松材线虫分子检测技术的比较分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年04期
Page:
128-132
Column:
研究论文
publishdate:
2007-04-20

Article Info:/Info

Title:
Comparative Analyses on Three Kinds of Molecular Detection Technique of Bursaphelenchus xylophilus
Article ID:
1000-2006(2007)04-0128-05
Author(s):
WANG Yan13 JI Lei2 YU Ben-yuan2 LIN Mao-song2 YE Jian-ren1*
1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. College of Plant Protection Nanjing Agricultural University, Nanjing 210095, China; 3. Shanghai Station of Forest Pest Quarantine and Control, Shanghai 200072, China
Keywords:
Bursaphelenchus xylophilus Molecular detection RFLP Real-time PCR
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2007.04.029
Document Code:
A
Abstract:
A comparative study on molecular detection of Bursaphelenchus xylophilus was conducted in this paper. Specific amplification PCR, ITS-PCR-RFLP and real time PCR were used in molecular detection of B. xylophilus. According to detection time, detection accuracy and operation skills, this paper summarized following conclusions: (1) Specific amplification PCR is used widely up to now because of its simple operation and low costs; (2) ITS-PCR-RFLP can be used to identify physiological race, being more accurate than Specific amplification PCR, however it takes long time to operate, it can be used for differentiation of population of B. xylophilus and species identification; (3) Real-time PCR takes short time to operate, has a high accurate results, and it’s easy to operate. However it needs specific instruments which are very expensive. It should be better used in quarantine of B. xylophilus.

References

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Last Update: 2013-05-20